computer-assisted image analysis software nis- elements Search Results


99
Nikon 3d sim image reconstruction
Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy <t>(3D-SIM)</t> allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="250" height="auto" />
3d Sim Image Reconstruction, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Schkade Landhandel computer-assisted day reconstruction interview
Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy <t>(3D-SIM)</t> allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="250" height="auto" />
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Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy <t>(3D-SIM)</t> allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="250" height="auto" />
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Verlag GmbH medical image computing and computer-assisted intervention—miccai'98
Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy <t>(3D-SIM)</t> allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="250" height="auto" />
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Hamamatsu dvs video image processing unit
Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy <t>(3D-SIM)</t> allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="250" height="auto" />
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86
Stryker computer assisted navigation
Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy <t>(3D-SIM)</t> allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="250" height="auto" />
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Verlag GmbH spinger-verlag lncs series
Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy <t>(3D-SIM)</t> allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="250" height="auto" />
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Image Search Results


Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy (3D-SIM) allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in <xref ref-type=Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm. " width="100%" height="100%">

Journal: mBio

Article Title: The Polar Organizing Protein PopZ Is Fundamental for Proper Cell Division and Segregation of Cellular Content in Magnetospirillum gryphiswaldense

doi: 10.1128/mBio.02716-18

Figure Lengend Snippet: Localization of PopZ Mgr -GFP in M. gryphiswaldense . (A) Time-lapse microscopy of cells expressing PopZ Mgr -GFP ( popZ Mgr :: popZ Mgr -gfp strain). First row, bright-field; second row, fluorescence channel; third row, overlay of bright-field and GFP channel. PopZ Mgr localizes to both cell poles. In dividing cells, an additional spot appears at the cell division site (fourth and ninth frames, white arrowheads). Generation time during time-lapse series was approximately 4 to 5 h. Numbers indicate hours and minutes. (B) Demograph of cells expressing PopZ Mgr -GFP ( n = 642 cells). The appearance of the signal at midcell is marked with an arrowhead. (C) Structured illumination microscopy (3D-SIM) allows us to resolve two PopZ foci in close proximity at the division plane with a distance near the resolution limit of conventional epifluorescence microscopy (∼250 nm). Micrographs are maximum-intensity projections of z-stack images from representative FM4-64-stained dividing cells. First row, bright-field (left image) and GFP channel (right image); second row, FM4-64 channel (left image) and overlay of FM4-64 and GFP channel (right image). Insets are magnified xy, xz, and yz projections of PopZ foci (GFP channel) and division plane (FM4-64 channel). Note cells with PopZ foci present at the site of division (Ci) had already completed compartmentalization and separation of their membranes, whereas no foci were observed in cells that were still connected (Cii). A representative number of dividing cells was imaged ( n = 29 [additional cells are shown in Fig. S2 ]). All scale bars not indicated in the figure correspond to 3 μm.

Article Snippet: 3D-SIM image reconstruction was performed in NIS-Elements 5.01 (Nikon) using the “stack reconstruction” algorithm with the following parameter settings.

Techniques: Time-lapse Microscopy, Expressing, Fluorescence, Microscopy, Epifluorescence Microscopy, Staining

Effect of full implementation of SurePath/FocalPoint or ThinPrep LBC and  computer-assisted reading  (last phase) <xref ref-type= a " width="100%" height="100%">

Journal: British Journal of Cancer

Article Title: Cervical histology after routine ThinPrep or SurePath liquid-based cytology and computer-assisted reading in Denmark

doi: 10.1038/bjc.2015.339

Figure Lengend Snippet: Effect of full implementation of SurePath/FocalPoint or ThinPrep LBC and computer-assisted reading (last phase) a

Article Snippet: We compared the sensitivity and specificity of liquid-based cytology (LBC) and computer-assisted reading for SurePath/FocalPoint and ThinPrep with those of manually read conventional cytology in routine cervical screening in four Danish laboratories.

Techniques: Imaging